fig1

Figure 1. Characterization of SPEVs from Yorkshire boars. (A) TEM image of SPEVs. (B) NTA results showing that the semen-derived EVs were approximately 50-200 nm in diameter. (C) The isolated SPEVs expressed the EV markers Tsg101, Alix, CD9 and CD 63, and the negative marker Calnexin was not found in our isolated SPEV samples. D. Bar plot comparing the total sperm motility between the high-sperm-mobility (H) group and the low-sperm-mobility (L) group.