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Figure 5. Isolation and characterization of RNA from pbMEC and MAC-T EVs. (A, B) Representative Agilent 2100 Bioanalyzer data from pbMEC (A) or MAC-T (B) RNA from EVs isolated by UF-SEC-UC (right) and UC-SEC-UC (left). (C) Total amount of RNA from pbMECs and MAC-T EVs isolated with UF-SEC-UC or UC-SEC-UC. (D) qPCR of let7a-5p and miR-200c-3p from EVs RNA isolated with either UF-SEC-UC or UC-SEC-UC from pbMEC (left) or MAC-T (right). (C, D) Bars show mean and SD of three independent experiments. EVs: Extracellular vesicles; UC: ultracentrifugation; SEC: size exclusion chromatography; pbMEC: primary bovine mammary epithelial cells; UF: ultrafiltration.