fig4

Figure 4. Antagomir-155 loading, targeting and delivery. (A) mEVs transfected with antagomiR-155 (αmiR mEV) showed expression of miR-155 compared to mEV alone and mEVs transfected with Control Oligo-1 miR (cmiR mEV). (B) αmiR, which was transfected into luciferase-plasmid-transfected HEK293 cells, successfully neutralized miR-155 and increased downstream luciferase expression. The ratio of Gaussia Luciferase (GLuc) and Secreted Alkaline Phosphatase (SEAP) is reported. (C) Mouse spleen CD14+ monocyte antagomiR-155 expression increases indicating successful delivery of αmiR mEV into monocytes. NT: No mEV treatment; αmiR: antagomiR-155; cmiR: control miRNA. *P < 0.05, **P < 0.01.