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Figure 2. EV-Aβ₄₀ induce aberrant NPC differentiation. (A) IncuCyte Zoom analysis of NPC neurogenesis. ReN cells were exposed to EV-PBS, EV-Aβ₄₀, or Aβ₄₀ every other day for 14 days of differentiation. Representative images from DIV3, DIV7, and DIV14 were taken where pink indicates neurite extensions and yellow represents cell bodies. Quantification of IncuCyte images demonstrates the change in rate of (B) total cell-body cluster area, (C) total neurite branch points, and (D) the ratio of neurite branch points to cell-body area. Graphs represent the average of individual pictures taken in nine image grids over 14 days. Additionally, DIV3 corresponds to timepoint 0 on the graph, the first instance when images were taken of cells after they were plated and treated. EV: Extracellular vesicles; NPC: neural progenitor cell.