fig3

Figure 3. EV-Aβ₄₀ alter the morphology of NPC mitochondrial cristae. Representative TEM images of morphology of perinuclear mitochondria and cristae in (A) untreated NPCs or those treated with (B) EV-PBS, (C) EV-Aβ₄₀, or (D) Aβ₄₀ alone as a positive control for 24 h. Quantification of (E) crista number, (F) crista surface area, (G) crista density, (H) crista score, and (I) circularity index. Scale bar for representative images is 2 µm and zoom inserts are 800 nm. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. n = 30 images per group and 38-72 mitochondria per group. EV: Extracellular vesicles; NPC: neural progenitor cell; TEM: transmission electron microscopy.