fig7

Figure 7. Expression of SIRT3 and NFκB is affected upon exposure to Aβ₄₀. (A) Schematic of mitochondrially dependent SIRT3 function affecting NFκB activation in NPCs. Reduced SIRT3 expression inhibits the deacetylation of NFκB, which allows the acetylated form to translocate into the nucleus and induce downstream inflammatory responses. SIRT3 expression was probed for both (B) total mRNA expression and (C) total protein expression. (D) Representative western blots. (E) Confocal images of NPCs after 24 h of treatment stained for DAPI (blue) and p65 protein (red) indicate translocation of NFκB into the nucleus of cells in the Aβ₄₀ groups. Images were further quantified for (F) total NFκB cell fluorescence, (G) nuclear NFκB, and (H) the nuclear-to-cytoplasmic ratio of NFκB fluorescence. Bar graphs indicate mean ± SEM. Statistical analysis was performed using one-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001,n = 3 independent experiments per group. EV: Extracellular vesicles; NPC: neural progenitor cell; DAPI: 4’,6-diamidino-2-phenylindole; SEM: standard error of the mean.