fig1
Figure 1. (A) qRT-PCR analysis showing viral burden (PrM transcript levels) from SH-SY5Y recipient cells that were incubated with infectious neuronal exosomes treated at varying temperatures (of -80 °C, 4 °C, 12 °C, 23 °C, or 37 °C) and held overnight in 1 × chilled PBS (of pH 7). All temperatures are represented as degree Celsius. Closed circles, squares, triangles, inverted triangles, or rhombus represents viral loads detected in LGTV-infected SH-SY5Y recipient cells incubated with infectious exosomes treated at indicated temperatures. In panel A, each data point represents data from one independent culture well in a plate. Each treatment had five independent replicates. The mRNA levels of prM gene are normalized to human beta-actin mRNA levels. P values less than 0.05 or equal to the numbers shown on the graph are from Student’s t-test. There is no statistical difference between the data observed in
