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Figure 2. (A) qRT-PCR analysis showing viral loads (PrM transcript levels) from SH-SY5Y recipient cells incubated (for three days) with infectious neuronal exosomes, treated overnight at 4 °C in 1 × chilled PBS (as control) or with PBS enriched 0.1 M salt solutions [of NaCl, (NH₄)₂SO₄, CH₃COONa or MgSO₄], respectively. Closed circles, squares, triangles, inverted triangles or rhombus represents viral loads obtained from LGTV-infected SH-SY5Y recipient cells incubated with infectious exosomes treated with respective salts. In panel A, each data point represents data from one independent culture well in a plate. Each treatment had five independent replicates. The mRNA levels of prM gene are normalized to human beta-actin mRNA levels. P values less than 0.05 or equal to the numbers shown in the graph are from Student’s t-test. There is no statistical difference between PBS to NaCl/CH3COONa, or NaCl to CH3COONa group. Immunoblotting analysis is shown for proteins NS1 (B) or CD9 (C) and for all tested salts. Protein sizes are indicated in kilodaltons (kDa). Protein profile gel images in (B) and (C) are shown as loading controls.