fig6

Figure 6. (A) qRT-PCR analysis showing viral loads (PrM transcript levels) from HaCaT recipient cells that were incubated (for 3 days) with infectious tick exosomes, treated overnight at 4 °C and at different pH conditions (of 1.5, 4, 7, 9 or 11.5), respectively. All pH conditions were set in 1x PBS solution. Closed circles, squares, triangles, inverted triangles or rhombus represents viral loads obtained from LGTV-infected HaCaT cells incubated with infectious exosomes treated at indicated pH. In panel A, each data point represents data from one independent culture well in a plate. Each treatment had five independent replicates. The mRNA levels of prM gene are normalized to human beta-actin mRNA levels. P values less than 0.05 or equal to the numbers shown in the graph are from Student’s t-test. There is no statistical difference between groups of pH 1.5 and 4, or 7 to 9 and 11.5. Immunoblotting analysis is shown for proteins NS1 or CD63 (B) and for all tested pH conditions. Protein sizes are indicated in kilodaltons (kDa). Protein profile gel image in (B) is shown as the loading control.