fig1

Figure 1. An experimental workflow for the isolation of EVs and the use of ELISA to analyze cytokines levels of EXO-NET EVs. (A) Size exclusion chromatography for EV isolation, referring to SEC-EVs in the following text; (B) Bead-based immunoaffinity capture (EXO-NET), referring to EXO-NET EVs in the following text; (C) ELISA for IL-6, IL-8, IL-10, and IL-1β for EXO-NET EVs from 12 non-periodontitis and 20 periodontitis patients. SN: Supernatant.