fig4

Figure 4. sEV miRNA characterization and next-generation sequencing demonstrated all four sEV miRNA isolation methodological approaches were successful (four technical replicates were utilized (n = 4) for each of the four isolation methods). (A) Quality control (QC) results of sEV miRNA samples quantified as pg/mL of miRNA concentration; (B) Summary of next-generation sequencing (NGS): different types of RNA read counts identified; (C) Total number of bovine (Bos taurus) miRNA counts identified; (D) the Venn diagram of shared and unique miRNAs in each method compared to the blood plasma control; (E) the principal components analysis (PCA) plot illustrates all sEV miRNA samples cluster together and away from the blood plasma-derived miRNA control. UC: Ultracentrifugation; SEC: Size exclusion chromatography; UF: Ultrafiltration.